Influence of Acupuncture on Infertility in Rats with Polycystic Ovarian Syndrome

Zhang Wei Yi et al, Zhongguo Zhong Xi Yi Jie He Za Zhi 2009 (11) 973 Chinese Journal of Integrated Traditional and Western medicine
Studies of animal models with PCOS have elucidated some of the physiological effects of acupuncture on different aspects such as Insulin resistance, hormone levels and ovulation frequency. This study measured ovulation frequency after acupunture by counting the number of blastocysts that had implanted after acupuncture treatment compared to a control group. Acupuncture proved to be an effective treatment to increase the number of blastocysts.



Objective: To observe the effect and mechanism of acupuncture on infertility of rats with polycystic ovarian syndrome (PCOS).

Methods: PCOS rat model was induced by subcutaneous injection of oil solution of dehydroepiandrosterone (DHEA) in immature (24-day-old) female rats for continuous 20 days.Rats for control were given with same dose of oil for instead.PCOS rats were randomly divided into the model group untreated and the acupuncture group treated by needling acupoints of Guanyuan (CV4),Zhongji (CV3),Sanyinjiao (SP6) and Zigong (CX-CA1),15 min once a day for 5 continuous days,starting from the 80th day after birth.All rats were sacrificed at terminal of the treatment,their uterus and bilateral ovaries were dissected for observation and blood levels of sex hormones were measured.

Results: Compared with the model group,the number of implanted blastocyte and blastocyte implantation rate were higher and the blood levels of testosterone (T) and estradiol (E2) were lower in the acupuncture group (P0.05);but the difference between groups in serum levels of follicular stimulating hormone,luteinzing hormone and progesterone were of statistical insignificance (P0.05).Moreover,the wet weight of ovary was lower and the equipotent diameter and area of glandular organ and cavity,area ratio of gland and the stroma,and mean thickness of endometria were higher in the acupuncture group than those in the control group (P0.05).

Conclusion: Acupuncture can conspicuously down-regulate the expressions of serum levels of T and E2,improve the development of ovaries and uterus,promote ovulation,enhance endometrial receptivity,and advance blastocyte implantation.